Protocols

All protocol files are listed below; they may also be found individually on each product page. Protocol files are available as PDF files. To view a PDF file, you will need install Adobe® Reader.

General RNA Protocols

Receiving RNA
Preparing RNA for Use
Reverse Phase Cartridge Desalting of RNA Oligonucleotides
Ethanol Precipitation of RNA Oligonucleotide
Deprotection Protocol
Thiol Reduction

siRNA Protocols

Accell siRNA Delivery
Basic siRNA Resuspension
siRNA Libraries Guidelines
siGENOME RTF siRNA Libraries
siGENOME RTF Optimization Kit 1
siGENOME RTF Optimization Kit 2
5X siRNA Buffer Recipe
siRNA Deprotection Buffer Recipe
Thiol Reduction

siRNA Library Protocols

siRNA Library Guidelines
siGENOME RTF siRNA Libraries
siGENOME RTF Optimization Kit 1
siGENOME RTF Optimization Kit 2
Reverse Transfection Protocol

shRNA Protocols

SMARTvector 2.0 Technical Manual

Transfection Protocols

DharmaFECT General Transfection
DharmaFECT Duo Transfection
DharmaFECT Duo Co-Transfection of miRIDIAN miRNA and Reporter Plasmid

Cell Line Specific Transfection Protocols

Currently undergoing revisions. Please refer to the DharmaFECT General Transfection protocol, which includes cell line-specific recommendations.