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Off-Target Effects: Disturbing the Silence of RNA interference (RNAi)
Jon Karpilow, PhD, and Allison O'Brien, PhD
Off-target effects occur when an siRNA is processed by the RNA-Induced Silencing Complex (RISC) and down-regulates unintended targets. As these changes in gene expression can lead to measurable phenotypes (i.e. false positives) it is of great importance to understand the mechanism behind off-targeting so that strategies can be developed to minimize their effects.
Read the entire Tech Review:
 Off Target Effects (414K)
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