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Using Mimics and Inhibitors for microRNA (miRNA) Functional Analysis
Rohaizah James, PhD
MicroRNAs (miRNAs) are naturally occurring short RNA molecules of about 22 nucleotide (nt) long that regulate gene expression by binding to target mRNA and repressing protein expression. Since their initial description in C. elegans in 1993, over 3500 miRNAs in almost 40 species have been identified. Subsequent functional and sequence analyses suggest that miRNAs play an important role in the regulation of many biological pathways.
The identification of their role in cancer development, cancer characterization and potential cancer therapy has elevated interest in studying the function of these small regulatory molecules. This Tech Note discusses the main steps in performing miRNA functional analysis . . .
Read the entire Tech Note:
 Using Mimics and Inhibitors (414K)
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