SMARTvector™ shRNA Lentiviral Particles – Your Long-Term, All-Access Pass For Targeted Gene Silencing

Figure 1
Click to enlarge		 The SMARTvector shRNA Lentiviral technology incorporates state-of-the-art design features that enable effective DNA-mediated RNAi.

The shRNA silencing constructs are designed using miRNA scaffold-specific attributes that are integrated into a lentiviral delivery platform for efficient processing via the endogenous RNAi pathway (Figure 1).

Lentigen Corporation has developed a production process that achieves high viral titers for gene silencing experiments suitable for dividing and non-dividing cell types, including difficult-to-transfect cells such as primary cells, neuronal cells and hematopoietic cells.

Together, these attributes greatly enhance the functionality and specificity of lentiviral-mediated RNAi and reduce the toxicity associated with low titer preparations.

  • Superior Dharmacon Design Algorithm
    • Utilization of a highly processed miRNA scaffold
    • Selection of functional and specific shRNA sequences
    • Expression of target-specific silencing constructs
Figure 2
Click to enlarge
  • Ease of Application
    • Packaged as ready-to-use lentiviral particles
    • Engineered for use with a broad range of cells
    • Modified with a self-inactivating 3' LTR (SIN vector)
    • Detectable expression of TurboGFP™ reporter (Figure 2)
    • Puromycin selectable marker for stable cell lines
Figure 3
Click to enlarge
  • Consistent production of high titer (>108) lentiviral particles
    • Lentigen-proprietary manufacturing process yields high-quality lentiviral stocks (Figure 3)
    • High efficiency transduction eliminates the necessity for drug selection
    • High titers minimize toxicity associated with low titer lentiviral preparations
    For more information on SMARTvector shRNA Lentiviral Particles visit here.

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