The SMARTvector 2.0 Lentiviral shRNA technology incorporates state-of-the-art design features that enable effective DNA-mediated RNAi
The shRNA silencing constructs are designed using miRNA scaffold-specific attributes that are integrated into a lentiviral delivery platform for efficient processing via the endogenous RNAi pathway.
SMARTvector 2.0 silencing constructs are delivered at high viral titers for gene silencing experiments suitable for dividing and non-dividing cell types, including difficult-to-transfect cells such as primary cells, neuronal cells and hematopoietic cells.
Together, these attributes greatly enhance the functionality and specificity of lentiviral-mediated RNAi and reduce the toxicity associated with low titer preparations.
Superior Dharmacon Design Algorithm
- Utilization of a highly processed, proprietary miRNA scaffold
- Rationally-designed, highly functional gene targeting sequences (Figure 1)
- Incorporation of bioinformatics strategies to reduce off-target gene knockdown events
Ease of Application
- Engineered for use with a broad range of cells (Figure 2)
- Modified with a self-inactivating 3' LTR (SIN vector)
- Detectable expression of turboGFP reporter
- Puromycin selection marker for creation of stable cell lines
Consistent production of high titer (>108 TU/mL lentiviral particles)
- Packaged as ready-to-use lentiviral particles
- High efficiency transduction eliminates the necessity for drug selection
- High titers minimize toxicity associated with low titer lentiviral preparations