siRNA and microRNA Library Screening
Complete high-throughput solutions are available for RNAi screening, including the most advanced and comprehensive genome-wide Dharmacon siRNA libraries, microRNA mimics, and microRNA inhibitors. These tools allow for comprehensive study of gene function and pathway analysis.
The portfolio of services available include:
- Cell culture optimization, banking, expansion and QA/QC of cell lines
- Assay development and validation: Optimization of delivery reagents and conditions for:
- End-point assays
- Small molecule-based conditional RNAi screening
- High content assays for multi-parametric readouts
- Screens using synthetic lethal strategies
- High-throughput siRNA and miRNA library screening:
- Genome-wide siRNA libraries for knockdown of each gene target in the human or mouse genomes
- Human, mouse and rat microRNA Mimic and Hairpin Inhibitor libraries for high-throughput functional studies
- Follow-up analysis of hits:
- Validation of target knockdown by qRT-PCR or branched DNA technologies
- Examination of hit specificity by global mRNA expression profiling
- Bioinformatics strategies for hit selection and stratification
microRNA Phenotypic Screening: Functional Characterization of microRNA Biology
MicroRNA phenotypic screening, accomplished using libraries of microRNA mimetics and inhibitors, permits the identification and validation of microRNA function in both the pathology of disease and the profiling of responses to therapeutic intervention. In addition, these neo-biomarkers are poised to create a bridge between pre-clinical observations and clinical efficacy and safety.
- Dharmacon miRIDIAN miRNA Mimics are chemically modified with the Dharmacon ON-TARGET pattern for exceptionally specific mature microRNA activity
- Dharmacon miRIDIAN miRNA Hairpin Inhibitors are designed using length and chemical modification strategies to enhance potency and longevity of inhibition
- Complete libraries of Mimics and Hairpin Inhibitors permit high-content phenotypic screening