Custom siRNA Synthesis

Overview Processing Options Modifications Related Products
  Ready to use (A4) 2’ ACE protected (A1) PAGE (C) HPLC In vivo In vivo HPLC
Purified     Purified Purified   Purified
2'ACE protected single- strands   2'ACE protected single strands        
Desalted Desalted   Desalted Desalted Desalted Desalted
Endotoxin tested         Endotoxin tested Endotoxin tested
Sodium counter-ion exchange         Sodium counter-ion exchange Sodium counter-ion exchange
Duplexed Duplexed   Duplexed Duplexed Duplexed Duplexed
Recommended for modified siRNA (dyes, etc.)     Recommended for modified siRNA (dyes, etc.) Recommended for modified siRNA (dyes, etc.)   Recommended for modified siRNA (dyes, etc.)
Recommended for in vivo use         Recommended for in vivo use Recommended for in vivo use

Description of siRNA Processing Terms

  • Ready to use: No further processing is required. Resuspend prior to use.
  • 2'-ACE protected: The RNA is protected at the 2' position with an acid-labile orthoester group (2'-ACE).
  • PAGE: The siRNA duplex has been purified by Polyacrylamide Gel Electrophoresis
  • HPLC: The duplex has undergone ion exchange HPLC purification and lyophilized
  • In vivo: The duplex has been processed by: counter ion (Na+) exchange, desalt, sterile filtration, endotoxin testing and lyophilized
  • Desalted:The siRNA duplex has been desalted by either ethanol precipitation (Ready to use) or reverse phase chromatography (PAGE).
  • Duplexed: The two complementary siRNA strands have been annealed to form a duplex.
  • Single-stranded:The RNA is provided with the sense and antisense strands in separate tubes.