We offer a complete portfolio of Thermo Scientific Dharmacon miRIDIAN microRNA experimental controls to optimize conditions and design relevant microRNA modulation experiments. Both positive and negative control molecules are provided for microRNA gain-of-function microRNA studies.
miRIDIAN microRNA Mimic Positive Controls
Successful microRNA functional studies begin with optimization of the assay in each cell line and/or type of interest. To optimize experimental conditions, we offer both housekeeping and endogenous positive controls for gain-of-function experiments using miRIDIAN Mimics.
Mimic Housekeeping Positive Controls
Highlights
- Employ the same structure and design as experimental miRIDIAN microRNA Mimics
- Target the 3' UTR (untranslated region) of the standard housekeeping genes, PPIB or GAPDH (Figure 1)
Applications
- Allow a clean, straightforward cleavage-based readout of mimic function
- Delivery and function can be evaluated to determine transfection conditions in each cell line/type
Mimic Endogenous Positive Control
Highlights
- Validated microRNA Mimic that targets Aldolase A in human, mouse, and rat
- Allows optimization of assay conditions by monitoring mimic function on an endogenous gene target (Aldolase A) with a conserved miR-122 binding site (Figure 2)
miRIDIAN microRNA Mimic Negative Controls
miRIDIAN microRNA Mimic Negative Control sequences are based on C. elegans miRNA for use as negative experimental controls in human, mouse, and rat cells. The Negative Controls have been analyzed by BLAST against all human, mouse and rat genomic sequences and miRNA sequences in the current miRBase Database.
Highlights
- Identical design and modifications as miRIDIAN microRNA Mimics
- No identifiable effects on tested miRNA function in human (Figure 3), mouse and rat cell lines
Applications
- Non-targeting mimics for use as controls in miRNA mimic experiments
- Distinguish between specific mimic activity and background effects
miRIDIAN microRNA Mimic Transfection Control
Highlights
- Non-targeting miRIDIAN Mimic labeled with Thermo Scientific Pierce Dy547 (ab/em max: 557/570 nm)
Applications
- No identifiable effects on tested miRNA function in multiple cell lines
- Transfection can be monitored and visualized by epifluorescence (Figure 4)