SMARTvector 2.0 Lentiviral shRNA Control Particles

Overview Related Products
SMARTvector 2.0 Positive Control Particles Species Catalog # Order Now
SMARTvector 2.0 GAPD Positive Control Particles H S-001000-01 Order
SMARTvector 2.0 Firefly Luciferase Positive Control Particles H, M, R S-006000-01 Order
SMARTvector 2.0 Negative Control Particles Species Catalog # Order Now
SMARTvector 2.0 Non-Targeting Negative Control Particles H, M, R S-005000-01 Order
SMARTvector 2.0 Empty Vector Transduction Efficiency Control Particles S-004000-01 Order

H=human, M=mouse, R=rat. X=1, 2, 3, 4 respectively

SMARTvector 2.0 Positive Controls

Highlights

  • SMARTvector positive control provided as packaged lentiviral particles
  • Consistent, potent silencing of non-essential, abundantly expressed housekeeping genes in human (Figure 1), mouse and rat cells
  • Targets Firefly Luciferase expressed from pGL2 and pGL3 (Promega Corp., Madison, WI)

Applications

  • Confirm transduction efficiency via turboGFP expression
  • Evaluate specific and potent silencing of housekeeping gene or expression of a fluorescent protein from a reporter plasmid

SMARTvector 2.0 Negative Controls

Highlights

  • SMARTvector negative control provided as packaged lentiviral particles
  • Validated to have minimal targeting of any known genes in human, mouse and rat genomes Empty vector expressing turboGFP (Evrogen, Moscow, Russia) and puromycin resistance to be used as transduction control

Applications

  • Confirm silencing specificity of gene-targeting SMARTvector shRNA Lentiviral Particles
  • Distinguish sequence-specific silencing from non-specific effects